Community for identifying and discussing microphotographs as well as microscopy techniques and microbial life in general. Researcher or microscope own

: microscopy : I built a scanning laser confocal microscope

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2021-05-19 09:29:07

Community for identifying and discussing microphotographs as well as microscopy techniques and microbial life in general. Researcher or microscope owner with some cool pics? Share it with us! Saw a weird critter in your pond samples the other day? Show us here and maybe we can sort out what it is!

Note: This is a public subreddit, so please avoid posting unpublished data unless you wouldn't mind it being distributed far and wide.

I hope this is OK, mods let me know if this is too off-topic. I recently made a scanning laser confocal microscope and thought it might be interesting to folks here. :)

My system is pretty much entirely 3D printed: the motion stage is using the open source OpenFlexure microscope stage, and the optical train has various optical components housed in 3D printed holders.

I managed to get some decent images off the microscope, and also some duds. As it turns out, there is a lot of nuance to processing the data that comes off the machine. It also takes a _very_ long time to scan: one of my larger scans took 63 continuous hours... and that's after a lot of optimization to bring the time down :)

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